
DOT Drug & Alcohol Testing
FMCSA-compliant testing for CDL drivers and DOT-regulated employees. Full compliance with 49 CFR Part 40.
What is DOT Testing?
DOT (Department of Transportation) drug and alcohol testing is federally mandated for employees in safety-sensitive transportation positions. It follows strict regulations under 49 CFR Part 40.
Unlike standard workplace testing, DOT testing has specific requirements for:
- Collection procedures and chain of custody
- Laboratory certification (SAMHSA)
- Medical Review Officer (MRO) review
- Clearinghouse reporting
- Specific 5-panel drug test (no variations)
Who Needs DOT Testing?
All prices are per person — includes DOT-compliant collection, lab analysis, MRO review, and Clearinghouse reporting.
DOT Testing Services

DOT Pre-Employment
Required before hiring any DOT-regulated employee.
- •DOT 5-panel urine drug test
- •FMCSA-compliant collection
- •SAMHSA-certified lab analysis
- •MRO review and verification

DOT Random Testing
Unannounced testing for DOT random pool members.
- •DOT 5-panel urine drug test
- •Random selection notification
- •Immediate scheduling required
- •Full chain of custody

DOT Post-Accident
Required after certain DOT-reportable accidents.
- •DOT 5-panel drug test
- •DOT breath alcohol test (BAT)
- •Must be completed within 8 hours (alcohol) / 32 hours (drugs)
- •Priority scheduling

DOT Reasonable Suspicion
When a trained supervisor observes signs of impairment.
- •DOT 5-panel drug test
- •Breath alcohol test available
- •Immediate scheduling
- •Supervisor documentation support

Return to Duty (RTD)
Required before returning to safety-sensitive duties after a violation.
- •Direct observation collection
- •DOT 5-panel drug test
- •SAP (Substance Abuse Professional) referral
- •MRO review and clearance

Follow-Up Testing
Ongoing testing after return-to-duty clearance.
- •Unannounced testing per SAP plan
- •Minimum 6 tests in first 12 months
- •DOT 5-panel drug test
- •Direct observation if required
Had a DOT Violation?
If you've had a positive drug test, refused a test, or violated DOT drug and alcohol regulations, you must complete the return-to-duty process before resuming safety-sensitive duties.
This includes evaluation by a Substance Abuse Professional (SAP), completing recommended treatment, and passing a return-to-duty test under direct observation.
Get Help NowDOT Random Testing Consortium
Owner-operators and small fleets can join our random testing consortium to meet DOT requirements without the hassle of managing your own program.
- •Meets FMCSA random testing requirements
- •Computer-generated random selections
- •Nationwide collection sites
- •Full compliance documentation
- •Clearinghouse reporting included
- •Affordable monthly rates

Need DOT-Compliant Testing?
We ensure full compliance with all FMCSA regulations. Schedule online for same-day appointments.
(317) 314-3757