DOT Drug Testing for Commercial Drivers

DOT Drug & Alcohol Testing

FMCSA-compliant testing for CDL drivers and DOT-regulated employees. Full compliance with 49 CFR Part 40.

What is DOT Testing?

DOT (Department of Transportation) drug and alcohol testing is federally mandated for employees in safety-sensitive transportation positions. It follows strict regulations under 49 CFR Part 40.

Unlike standard workplace testing, DOT testing has specific requirements for:

  • Collection procedures and chain of custody
  • Laboratory certification (SAMHSA)
  • Medical Review Officer (MRO) review
  • Clearinghouse reporting
  • Specific 5-panel drug test (no variations)

Who Needs DOT Testing?

CDL Truck Drivers
Interstate and intrastate commercial drivers
Bus Drivers
School bus and transit operators
Pipeline Workers
Natural gas and hazardous liquid pipeline employees
Railroad Workers
Train operators and dispatchers
Aviation Workers
Pilots, flight attendants, mechanics
Maritime Workers
Commercial vessel crew members

All prices are per person — includes DOT-compliant collection, lab analysis, MRO review, and Clearinghouse reporting.

DOT Testing Services

DOT Pre-Employment
MOST COMMON
$89/person

DOT Pre-Employment

Required before hiring any DOT-regulated employee.

  • DOT 5-panel urine drug test
  • FMCSA-compliant collection
  • SAMHSA-certified lab analysis
  • MRO review and verification
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DOT Random Testing
$89/person

DOT Random Testing

Unannounced testing for DOT random pool members.

  • DOT 5-panel urine drug test
  • Random selection notification
  • Immediate scheduling required
  • Full chain of custody
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DOT Post-Accident
$89/person

DOT Post-Accident

Required after certain DOT-reportable accidents.

  • DOT 5-panel drug test
  • DOT breath alcohol test (BAT)
  • Must be completed within 8 hours (alcohol) / 32 hours (drugs)
  • Priority scheduling
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DOT Reasonable Suspicion
$89/person

DOT Reasonable Suspicion

When a trained supervisor observes signs of impairment.

  • DOT 5-panel drug test
  • Breath alcohol test available
  • Immediate scheduling
  • Supervisor documentation support
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Return to Duty (RTD)
$425/person

Return to Duty (RTD)

Required before returning to safety-sensitive duties after a violation.

  • Direct observation collection
  • DOT 5-panel drug test
  • SAP (Substance Abuse Professional) referral
  • MRO review and clearance
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Follow-Up Testing
$89/person

Follow-Up Testing

Ongoing testing after return-to-duty clearance.

  • Unannounced testing per SAP plan
  • Minimum 6 tests in first 12 months
  • DOT 5-panel drug test
  • Direct observation if required
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Had a DOT Violation?

If you've had a positive drug test, refused a test, or violated DOT drug and alcohol regulations, you must complete the return-to-duty process before resuming safety-sensitive duties.

This includes evaluation by a Substance Abuse Professional (SAP), completing recommended treatment, and passing a return-to-duty test under direct observation.

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DOT Random Testing Consortium

Owner-operators and small fleets can join our random testing consortium to meet DOT requirements without the hassle of managing your own program.

  • Meets FMCSA random testing requirements
  • Computer-generated random selections
  • Nationwide collection sites
  • Full compliance documentation
  • Clearinghouse reporting included
  • Affordable monthly rates
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DOT Random Testing Consortium

Need DOT-Compliant Testing?

We ensure full compliance with all FMCSA regulations. Schedule online for same-day appointments.

(317) 314-3757